Synopsis: DO ANJANE is the story of a man who after an accident looses his memory. Then one day when he is watching a movie, he is astonished as he feels that the actress of the movie is related to him, and his whole life reels back to him.

Calcutta based Amit Roy (Amitabh) is married to Rekha (Rekha) who aspires to be an actress. However it is only after the marriage that Amit finds out that he is not the ideal man for Rekha, and both make compromises and continue living with each other. Soon, a son, Sumit, is born and it appears that Rekha is now destined to live the life of a middle-class housewife, far away from her desire to be an actress.

Then Ronjit Malik (Prem Chopra) enters their lives, Rekha's desire to make it as an actress is rekindled. They decide to travel to Bombay to further her career; this journey ends tragically, as a drunken Amit falls from the train, resulting in his death.

With nothing to hold her back, Rekha admits Sumit in a boarding school and chases her dream. She goes on to be the reigning leading actress of Bengali cinema. Then a producer introduces her to Naresh Dutt, a Bombay-based financier, who would like her to act in his movie titled "Do Anjaane", to which Rekha agrees. Then a series of events follow which lead both Rekha and Ronjit to question whether Amit is really dead or has his restless ghost has come to haunt them.